Web usability is a broad term. It encompasses HCI (Human Computer Interaction), information architecture, and usability testing,
among other things. Accessibility is also an integral part of usability. It has unique concerns, but
there is considerable overlap between the two. What makes a site more accessible often makes it more usable for everyone.
There are various usability schools of thought.
You may find you agree more with one approach than others. Whatever you do, don't give up on it. It's vital you keep focused on usability throughout the entire life cycle of what you create for the Web. Usability is a frame of mind.
How well a Web site prospers (or how quickly it perishes) may be directly attributed to its usability.
If a site is easy to use and accomplishes users' goals, it will more likely become popular and draw repeat visitors. Usability makes so much business sense, it should be common sense!
Many usability resources are available online and in print. You may start with those,
or find a firm to conduct your usability research. The latter is best if you lack the time, knowledge,
or interest.
There is a growing number of knowledgeable usability experts out there today. Whether you conduct
usability research "in-house" or with the help of consultants, the information
you uncover will be an invaluable long term investment.